Free tool · no download needed

Peptide reconstitution
calculator.

Enter your vial and the bacteriostatic water you are adding, and this works out the concentration and exactly where to draw to on a U-100 insulin syringe. Same maths the app runs — use it here without installing anything.

12.5units to draw
Concentration5 mg/mL
Volume per dose0.05 mL
Doses per vial40

How the maths works

Three steps, and the calculator shows all three so you can check it against your own arithmetic.

1 · Concentration

The peptide in the vial divided by the water you add. A 10 mg vial with 2 mL of bacteriostatic water is 5 mg/mL. Adding more water does not change how much peptide is in the vial — it only changes how much liquid each dose comes in.

2 · Volume to draw

Your dose divided by the concentration. A 250 mcg dose at 5 mg/mL is 0.25 mg ÷ 5 mg/mL = 0.05 mL.

3 · Syringe units

On a U-100 insulin syringe, 100 units is 1 mL, so every unit mark is 0.01 mL. That 0.05 mL draw is the 5-unit mark. The units are volume, not compound — the same 5-unit draw is a completely different dose if you reconstituted the vial differently.

Common vial and water combinations

A starting point for the numbers people ask about most. Enter your own above rather than assuming one of these matches your vial.

VialWaterConcentration250 mcg dose500 mcg dose
5 mg1 mL5 mg/mL5 units10 units
5 mg2 mL2.5 mg/mL10 units20 units
10 mg1 mL10 mg/mL2.5 units5 units
10 mg2 mL5 mg/mL5 units10 units
10 mg3 mL3.33 mg/mL7.5 units15 units
15 mg3 mL5 mg/mL5 units10 units
20 mg2 mL10 mg/mL2.5 units5 units

Less water is not a stronger dose. The vial holds what it holds. Reconstituting with less water means each dose is a smaller, harder-to-measure draw — it does not give you more compound.

Reconstitution questions

How do I calculate peptide reconstitution?

Divide the peptide in the vial by the bacteriostatic water you add to get the concentration. Divide your dose by that concentration to get the volume to draw. On a U-100 insulin syringe each 0.01 mL is one unit mark, so a 0.20 mL draw is the 20-unit line. The calculator above does all three steps and shows you the mark.

How much bacteriostatic water should I add to a 10mg vial?

There is no single right answer — the water volume sets the concentration, not the total dose. More water makes each dose a larger, easier-to-measure draw; less water makes the vial last longer in the fridge. 2 mL into a 10 mg vial gives 5 mg/mL, which puts a 250 mcg dose at the 5-unit mark. Enter your own numbers above to see where your dose lands.

What do the units on an insulin syringe mean?

On a U-100 syringe, 100 units is 1 mL. Each unit mark is 0.01 mL. The units are a volume measurement, not a dose of peptide — the same 10-unit draw is a different amount of compound depending on how you reconstituted the vial.

Why can't I convert IU to mg?

IU measures biological activity, not mass, and the conversion factor is different for every compound — there is no universal number. A vial labelled in IU can only be dosed in IU. The calculator locks both menus to one family for exactly this reason, and the app does the same.

Does bacteriostatic water expire once the vial is mixed?

Bacteriostatic water contains 0.9% benzyl alcohol as a preservative and is typically discarded 28 days after first puncture. Reconstituted peptide stability varies by compound. Follow the guidance that came with your vial and your prescriber's direction — this calculator does arithmetic, it does not advise on storage.

Is this reconstitution calculator free?

Yes, entirely, with nothing to install and no account. The same calculator is built into the BioHack Track app for iPhone and Android, which also tracks the vial's remaining doses as you log.

Track the vial, not just
the arithmetic.

The app runs this same calculation, then remembers it — every dose you log comes off the vial, so you always know how many are left and when to reorder. Free, unlimited.